If you use 6-well or 12-well plates, total volume of the medium should be 3ml and 1.5ml, respectively, and decrease the amount of each reagent accordingly.2. One hour prior to the transfection, change to medium containing 25M chloroquine (from x1000 stock in PBS, stored at -20C). (Chloroquine can be omitted, but increases efficiency about x2) to a final volume of 500 l. Plaquenil and ranitidine Plaquenil avkare This protocol is a modified version of a published method4, in which calcium phosphate–based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney cells. I have another question regarding calcium phosphate transfections. I am planning to do another this week, but I would like to compare standard CaPO4 transfection efficiencies w/those of modified techniques. I've read several articles suggesting a glycerol shock and/or adding chloroquine to the media. Aralen, chloroquine phosphate, USP, is a 4-aminoquinoline compound for oral administration. It is a white, odorless, bitter tasting, crystalline substance, freely soluble in water. Aralen is an antimalarial and amebicidal drug. Add this mixture directly to the cells dropwise through the medium. As long as the cells are alive, they produce proteins. When you are ready, add 500 l of 2x HBS dropwise while gently mixing. Make sure you evenly sprinkle the droplet over the entire area.5. For secreted protein, you can change media once (at day 3 or 4, save the collected sup) and give it another 3-4 days of culture. Chloroquine transfection calcium phosphate Transient Transfection of 293T cells, Glycerol shock or chloroquine? - Transfection and Transduction Plaquenil antimalarialPlaquenil risk of deathDrug treatment of chloroquine resistant malaria CHLOROQUINE PHOSPHATE, USP DESCRIPTION ARALEN, chloroquine phosphate, USP, is a 4-aminoquinoline compound for oral administration. It is a white, odorless, bitter tasting, crystalline substance, freely soluble in water. ARALEN is an antimalarial and amebicidal drug. CHLOROQUINE PHOSPHATE, USP. Aralen - FDA prescribing information, side effects and uses. Evaluation of Methods for Transient Transfection of a.. Transfection can be carried out using calcium phosphate i.e. tricalcium phosphate, by electroporation, by cell squeezing or by mixing a cationic lipid with the material to produce liposomes that fuse with the cell membrane and deposit their cargo inside. Transfection can result in unexpected morphologies and abnormalities in target cells. The Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation. How it works The calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium phosphate-DNA precipitate. We are working on gene transfection by Calcium Phosphate method but after 24h when I want to change the cell culture medium of my test and contro cells, I see that tests are not as normal as.